Brian D. Wade

Microbial Ecology and Evolution

Curriculum Vitae

PDF of CV

RESEARCH INTERESTS


Microbial Ecology and Evolution, Microbiogeology, Molecular Genetic Techniques, Astrobiology


HIGHER EDUCATION

Bachelor of Arts, Major in Biology, Minor in Chemistry, University of Louisville, Louisville, Kentucky, 1999


RESEARCH EXPERIENCE

Graduate Research Assistant, Michigan State University, Crop and Soil Sciences Department, 2003/4, 2006 – present

Research: Evolutionary adaptations of the bacterium Escherichia coli to stressors (2006 – present)
Research: Molecular genetics of the psychrophilic bacterium Psychrobacter arcticus (2003 and 2004)

Graduate Research Assistant, Arizona State University, Microbiology Program, 2000
2003
Research: Cyanobacterial ecology and microbiogeology of modern microbialites

Research Associate, Michigan State University, Center for Microbial Ecology, 1999
and 2000
Research: Bacterial ecology and microbiogeology of continental deep subsurface rocks

Laboratory Technician, University of Louisville, Biology Department, 1996
1999
Responsibilities: Collected and processed aquatic algal samples for analyses and performed field experiments


TEACHING EXPERIENCE


Graduate Teaching Assistant, Michigan State University, Biological Sciences Program, Spring 2008

Course: Honors Cell and Molecular Biology Laboratory

Graduate Teaching Assistant, Marine Biological Laboratory, Woods Hole, Massachusetts, Summer 2003

Course: Microbial Diversity

Graduate Teaching Assistant, Arizona State University,
Microbiology Program, 2000 2003
Course: Techniques in Molecular Biology Laboratory (x 2)
Course: Science Service Learning, Microbiology Focus


PUBLIC OUTREACH


Teaching Assistant: The Unseen Microbial World, Michigan State University, “Grandparents University”, June 25 and 27, 2008


Guide: Geomicrobiology Research at Arizona State University, Spring 2002


Representative: Astrobiology Program, Arizona State University, “See ASU”, March 29 and 30, 2002


Representative: Geomicrobiology Laboratory, Arizona State University, “Astrobiology Day”, September 26, 2000



VOLUNTEER SERVICE


Volunteer Sample Collector, Kentucky Watershed Watch, 1998 and 1999

Service: Obtained aquatic samples throughout the year for environmental monitoring of streams in Kentucky


PUBLICATIONS


Elser, J. J., J. H. Schampel, F. Garcia-Pichel,
B. D. Wade, V. Souza, L. Eguiarte, A. Escalante, J. D. Farmer. 2005. Effects of phosphorus enrichment and grazing snails on modern stromatolitic microbial communities. Freshwater Biology 50: 1808 – 1825.

Carson, E., T. Dowling, L. E. Eguiarte, J. Elser, A. Escalante, J. Farmer, F. Garcia-Pichel, P. Roopnarine, V. Souza, C. Tang,
B. Wade. 2004. Cuatro Ciénegas: A living lab for the study of present and past biospheres. In Under the Desert: The Mysterious Waters of Cuatro Ciénegas, edited by G. Badino, T. Bernabei, A. De Vivo, I. Giulivo, G. Savino, published by Edizioni Tintoretto, Italy, 88 95.

Garcia-Pichel, F., F. A. Al-Horani, J. D. Farmer, R. Ludwig,
B. D. Wade. 2004. Balance between microbial calcification and metazoan bioerosion in modern stromatolitic oncolites. Geobiology 2: 49 57.

Wade
, B. D., F. Garcia-Pichel. 2003. Evaluation of DNA extraction methods for molecular analyses of microbial communities in modern calcareous microbialites. Geomicrobiology Journal 20: 549 561.

Garcia-Pichel, F.,
B. D. Wade, J. D. Farmer. 2002. Jet-suspended, calcite-ballasted cyanobacterial waterwarts in a desert spring. Journal of Phycology 38: 420 428.

Wade
, B. D., F. H. Crocker, M. S. Roberts, F. J. de Bruijn, T. L. Marsh. Stratal interfaces as astrobiological targets: An example from shale and sandstone strata deep beneath the southeastern San Juan Basin in northwestern New Mexico, USA. Writing manuscript for Geochemical Transactions.

Wade
, B. D., M. J. Rothrock Jr. Halothece ribotypes dominate the cyanobacterial community of gypsiferous endoevaporites at multiple locales. Writing manuscript for Saline Systems.


CONFERENCE ABSTRACTS


Wade
, B. D., F. Garcia-Pichel. 2003. Evaluation of DNA extraction methods for molecular analyses of microbial communities in modern calcareous microbialites. Aquatic Sciences Meeting of the American Society of Limnology and Oceanography. Book of Abstracts, p. 134 (# 204).

Wade
, B. D., F. Garcia-Pichel. 2003. A molecular analysis of calcifying cyanobacteria that build dendroidal oncolites in the Mezquites River of the Cuatro Ciénegas Basin. General Meeting of the NASA Astrobiology Institute, Tempe, Arizona, USA. Book of Abstracts, p. 230 (# 12993).

Wade
, B. D., M. J. Rothrock Jr., F. Garcia-Pichel. 2003. A comparison of 16S rDNA sequences from cyanobacteria that build thalassic teepees and athalassic mats in evaporitic, gypsiferous environments. General Meeting of the NASA Astrobiology Institute, Tempe, Arizona, USA. Book of Abstracts, p. 356 (# 12994).

Farmer, J. D.,
B. D. Wade, F. Garcia-Pichel, V. Routt. 2003. Morphogenesis, microbial fossilization processes and biomolecular community analysis of modern carbonate oncolites, Mezquites River, Cuatro Ciénegas, Mexico. General Meeting of the NASA Astrobiology Institute, Tempe, Arizona, USA. Book of Abstracts, p. 537 (# 13006).

Elser, J. J., J. H. Schampel, J. Watts, F. Garcia-Pichel,
B. D. Wade, J. D. Farmer, V. Souza, L. E. Eguiarte. 2003. Effects of grazers and PO4 enrichment on biomass, C:N:P stoichiometry and microbial community structure of oncoid stromatolites at Cuatro Ciénegas, Coahuila, Mexico. General Meeting of the NASA Astrobiology Institute, Tempe, Arizona, USA. Book of Abstracts, p. 213 (# 12864).

Garcia-Pichel, F., F. Al-Horani, R. Ludwig,
B. D. Wade. 2003. Direct assessment of calcification rates in modern stromatolitic oncolites. General Meeting of the NASA Astrobiology Institute, Tempe, Arizona, USA. Book of Abstracts, p. 536 (# 13000).

Rothrock Jr., M. J.,
B. D. Wade, F. Garcia-Pichel. 2002. Molecular analysis of cyanobacteria-dominated evaporitic gypsum teepees. General Meeting of the American Society for Microbiology, Salt Lake City, Utah, USA. Final Program, p. 108 (# N-109).




Current as of December 2008